Radioiodination of Cellex®

详细

The possibility of labeling Cellex® with ¹²³I was examined. Labeling was performed by oxidative iodination in the presence of Chloramine-T and Iodogen. The optimum labeling conditions were found. The target product with the radiochemical purity higher than 95% was isolated from the reaction mixture by column gel chromatography. The label distribution between constituent proteins of different molecular masses was determined by gel electrophoresis.