Variation of Spectral Characteristics of Coelenteramide-Containing Fluorescent Protein from Obelia Longissima Exposed to Dimethyl Sulfoxide
- Авторы: Petrova A.S.1,2, Alieva R.R.1, Belogurova N.V.1, Tirranen L.S.1,2, Kudryasheva N.S.1,3
-
Учреждения:
- Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences
- Krasnoyarsk State Agrarian University
- Siberian Federal University
- Выпуск: Том 59, № 4 (2016)
- Страницы: 562-567
- Раздел: Article
- URL: https://ogarev-online.ru/1064-8887/article/view/237183
- DOI: https://doi.org/10.1007/s11182-016-0806-8
- ID: 237183
Цитировать
Аннотация
Effect of dimethyl sulfoxide (DMSO), a widespread biomedical agent, on spectral-luminescent characteristics of coelenteramide-containing fluorescent protein – discharged obelin – is investigated. Contributions of violet and blue-green spectral components to fluorescence of discharged obelin are elucidated and characterized at different photoexcitation energies. Dependences of these contributions on the DMSO concentration are presented. Spectral changes are related to the destructive effect of DMSO on fluorescent protein and decreasing efficiency of proton transfer to electronically excited states of fluorophore.
Об авторах
A. Petrova
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences; Krasnoyarsk State Agrarian University
Автор, ответственный за переписку.
Email: aileenpetpova@gmail.com
Россия, Krasnoyarsk; Krasnoyarsk
R. Alieva
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences
Email: aileenpetpova@gmail.com
Россия, Krasnoyarsk
N. Belogurova
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences
Email: aileenpetpova@gmail.com
Россия, Krasnoyarsk
L. Tirranen
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences; Krasnoyarsk State Agrarian University
Email: aileenpetpova@gmail.com
Россия, Krasnoyarsk; Krasnoyarsk
N. Kudryasheva
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences; Siberian Federal University
Email: aileenpetpova@gmail.com
Россия, Krasnoyarsk; Krasnoyarsk
Дополнительные файлы
