Some characteristics of transgenic clones of mouse R1 line embryonic stem cells


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Transgenic clones of mouse embryonic stem cells of the R1 line were received by transfection of plasmid linear vectors. The changes in the transgene structure during its integration into the genome of the target cells were investigated. Displacements were found on the flanks of the integrated transgene. It was found that multicopy tandem structures are formed in head–tail orientation at the transgene integration. It was noted that the number of copies of the integrated transgenes varies considerably, but the introduction of DNA fragments isolated from the nuclear envelopes into the flanks of the transgene normalizes the number of its copies.

Авторлар туралы

S. Drozd

Kol’tsov Institute of Developmental Biology

Хат алмасуға жауапты Автор.
Email: sdrozd@yandex.ru
Ресей, ul. Vavilova 26, Moscow, 119334

S. Surkov

Kol’tsov Institute of Developmental Biology

Email: sdrozd@yandex.ru
Ресей, ul. Vavilova 26, Moscow, 119334

M. Glazkov

Kol’tsov Institute of Developmental Biology

Email: sdrozd@yandex.ru
Ресей, ul. Vavilova 26, Moscow, 119334

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML

© Pleiades Publishing, Inc., 2016