Phagocytic activity of neutrophil blood granulocytes in children with chronic non-specific lung diseases with post-inflammatory pneumofibrosis
- 作者: Evseeva G.P.1, Knizhnikova E.V.1, Abdulina N.O.1, Pichuginа S.V.1, Chaika M.S.1
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隶属关系:
- Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration
- 期: 卷 27, 编号 4 (2024)
- 页面: 1001-1008
- 栏目: SHORT COMMUNICATIONS
- URL: https://ogarev-online.ru/1028-7221/article/view/267919
- DOI: https://doi.org/10.46235/1028-7221-16811-PAO
- ID: 267919
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One of the reasons for the development of chronic forms of lung diseases and pneumofibrosis as a pathological outcome of this inflammation may be an insufficient immune response. The key position belongs to neutrophilic granulocytes. Given the importance and renewed interest in neutrophils as tools for regulating immunity, leading to the progression of the fibrous process in children with CKD is an urgent problem. The purpose of the study was to study the phagocytic activity of blood neutrophils as an important component of innate immunity in patients with CNLD. Seventy children with CNLD with focal or segmental post-inflammatory pulmonary fibrosis were examined. Of these, 27 were children with progression of PPF (main group) and 43 children with PPF without progression (comparison group). The controls were the indicators of 23 healthy children, comparable by gender and age. The studies were carried out during clinical remission. The phagocytic activity of neutrophils (PНAN), phagocytic number (PN), spontaneous and stimulated NBT test were determined. The stimulation index of the NBT test (NBTst-NBTsp) was calculated. The mitochondrial membrane potential was determined using a BD FACS Calibur cytometer (USA) (BD Pharmigen, USA).
PN in children with chronic heart disease with progressive PF was significantly reduced in comparison with patients in the comparison group and with the control group. The PHAN in children with CKD in both groups was within the standard values, however, it was lower than in the control group. The activity of the spontaneous HBT test in children of the main group was 1.7 times higher than in children in the comparison group and 3 times higher than in children from the control group. However, the stimulation index in the main group was significantly lower than in the control group. An increase in cells with a reduced membrane potential of granulocyte mitochondria was revealed in children of the main group compared with the control group. Children with CKD with the progression of fibrosis processes develop changes in the functional and metabolic activity of peripheral blood neutrophils typical for an inflammatory reaction, characterized by an increase in oxygen-dependent and a decrease in phagocytic cell activity.
作者简介
G. Evseeva
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration
编辑信件的主要联系方式.
Email: evceewa@yandex.ru
PhD, MD (Medicine), Deputy Director on Scientific Work, Chief Research Associate, Group of Health and Environmental Problems of Mother and Child Health, Research Institute of Maternity and Childhood Protection
俄罗斯联邦, KhabarovskE. Knizhnikova
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration
Email: evceewa@yandex.ru
Postgraduate Student, Research Institute of Maternity and Childhood Protection
俄罗斯联邦, KhabarovskN. Abdulina
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration
Email: evceewa@yandex.ru
Postgraduate Student, Research Institute of Maternity and Childhood Protection
俄罗斯联邦, KhabarovskS. Pichuginа
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration
Email: evceewa@yandex.ru
PhD (Medicine), Senior Research Associate, Group of Clinical Immunology and Endocrinology, Pulmonologist, Research Institute of Maternity and Childhood Protection
俄罗斯联邦, KhabarovskM. Chaika
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration
Email: evceewa@yandex.ru
Research Associate, Group of Clinical Immunology and Endocrinology, Research Institute of Maternity and Childhood Protection
俄罗斯联邦, Khabarovsk参考
- Ананьева Л.П., Авдеев С.Н., Тюрин И.Е., Лила А.М., Загребнева А.И., Маслянский А.Л., Терпигорев С.А, Степанян И.В., Лашина Е.Л., Васильева О.С., Лукина О.С., Першина Е.С., Клименко А.А., Шостак Н.А., Насонов Е.Л. Хронические фиброзирующие интерстициальные заболевания легких с прогрессирующим фенотипом // Научно-практическая ревматология, 2020. Т. 58, № 6. С. 631-636. [Ananieva L.P., Avdeev S.N., Tyurin I.Е., Lila А.М., Zagrebneva А.I., Maslyanskiy А.L., Terpigorev S.А., Stepanyan I.V., Lashina E.L., Vasilieva О.S., Lukina О.S., Pershina Е.S., Klimenko А.А., Shostak N.А., Nasonov Е.L. Chronic fibrosing interstitial lung disease with progressive phenotype. Nauchno-prakticheskaya revmatologiya = Rheumatology Science and Practice, 2020, Vol. 58, no. 6, pp. 631-636. (In Russ.)]
- Долгушин И.И., Мезенцева Е.А., Савочкина А.Ю., Кузнецова Е.К. Нейтрофил как «многофункциональное устройство» иммунной системы // Инфекция и иммунитет, 2019. Т. 9, № 1. С. 9-38. [Dolgushin I.I., Mezentseva E.A., Savochkina A.Yu., Kuznetsova E.K. Neutrophil as a multifunctional relay in immune system. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2019, Vol. 9, no. 1, pp. 9-38. (In Russ.)] doi: 10.15789/2220-7619-2019-1-9-38.
- Евсеева Г.П., Лебедько О.А., Супрун С.В., Яковлев Е.И., Кудерова Н.И., Книжникова Е.В., Телепнева Р.С., Пивкина Т.В. Оценка мембранного потенциала митохондрий гранулоцитов у детей с хроническими воспалительными заболеваниями легких // Якутский медицинский журнал, 2020. Т. 72, № 4. С 10-13. [Evseeva G.P., Lebed’ko O.A., Suprun S.V., Yakovlev E.I., Kuderova N.I., Knizhnikova E.V., Telepneva R.S., Pivkina T.V. Estimation of the mitochondria membrane potential of granulocytes in children with chronic inflammatory diseases of the lungs. Yakutskiy meditsinskiy zhurnal = Yakut Medical Journal, 2020, Vol. 72, no. 4. pp. 10-13. (In Russ.)]
- Козлов В.К., Лебедько О.А., Пичугина С.В., Сиротина-Карпова М.С., Евсеева Г.П., Гандуров С.Г. Актуальные вопросы хронических неспецифических заболеваний легких у детей // Вопросы практической педиатрии, 2019. Т. 14, № 3. С. 22-31. [Kozlov V.K., Lebedko O.A., Pichugina S.V., Sirotina-Karpova M.S., Evseeva G.P., Gandurov S.G. Relevant problems of chronic obstructive pulmonary diseases in children. Voprosy prakticheskoy pediatrii = Clinical Practice in Pediatrics, 2019, Vol. 14, no. 3, pp. 22-31. (In Russ.)]
- Кудрявцев И.В., Головкин А.С., Зурочка А.В., Хайдуков С.В. Современные методы и подходы к изучению апоптоза в экспериментальной биологии // Медицинская иммунология, 2012, Т. 14, № 6. С. 461-482. [Kudriavtsev I.V., Golovkin A.S., Zurochka A.V., Khaidukov S.V Modern technologies and approaches to the study of apoptosis in experimental Biology. Meditsinskaya immunologiya = Medical Immunology (Russia), 2012, Vol. 14, no. 6, pp. 461-482. (In Russ.)]
- Нестерова И.В., Колесникова Н.В., Чудилова Г.А., Ломтатидзе Л.В., Ковалева С.В., Евглевский А.А., Нгуен Т.З.Л. Новый взгляд на нейтрофильные гранулоциты: переосмысление старых догм. Часть 1 // Инфекция и иммунитет, 2017. Т. 7, № 3. С. 219-230. [Nesterova I.V., Kolesnikova N.V., Chudilova G.A., Lomtatidze L.V., Kovaleva S.V., Evglevsky A.A., Nguyen T.D.L. The new look at neutrophilic granulocytes: rethinking old dogmas. Part 1. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2017, Vol. 7, no. 3, pp. 219-230. (In Russ.)] doi: 10.15789/2220-7619-2017-3-219-230.
- Хоринко А.В., Амарантов Д.Г., Косарева П.В. Роль нарушения клеточно-матриксных взаимодействий в патогенезе прогрессирования фиброза легких // Журнал анатомии и гистопатологии, 2016. Т. 5, № 3. С. 84-89. [Khorinko A.V., Amarantov D.G., Kosareva P.V. The role of the disorders of cell matrix interactions in the pathogenesis of pulmonary fibrosis progression. Zhurnal anatomii i gistopatologii = Journal of Anatomy and Histopathology, 2016, Vol. 5, no. 31, pp. 84-89. (In Russ.)]
- Шен С.-Ф., Гуан В.-С., Ду Дж.-Ф., Пузырева Л.В. Нарушение апоптоза нейтрофилов при сепсисе // Инфекция и иммунитет, 2018. Т. 8, № 2. С. 119-126. Shen X.-F., Guan W.-X., Du J.-F., Puzyreva L.V. Disturbance of an apoptosis of neutrophils at a sepsis. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2018, Vol. 8, no. 2, pp. 119-126. (In Russ.)] doi: 10.15789/2220-7619-2018-2-119-126.
- Bakalović G., Bokonjić D., Mihajlović D., Čolić M., Mališ V., Drakul M., Tomić S., Jojić I., Rakočević S., Popović D., Kozić L., Vasiljević M., Bekić M., Mašić S., Ljuboja O. Dysfunctions of neutrophils in the peripheral blood of children with cystic fibrosis. Biomedicines, 2023, Vol. 11, no. 6, 1725. doi: 10.3390/biomedicines11061725.
- Barkas G.I., Daniil Z., Kotsiou O.S. The role of small airway disease in pulmonary fibrotic diseases. J. Pers. Med., 2023, Vol. 13, no. 11, 1600. doi: 10.3390/jpm13111600.
- Castanheira F.V.S., Kubes P. Neutrophils and NETs in modulating acute and chronic inflammation. Blood, 2019, Vol. 133, no. 20. pp. 2178-2185.
- Ding L., Yang J., Zhang Ch., Zhang X., Gao P. Neutrophils modulate fibrogenesis in chronic pulmonary diseases. Front. Med., 2021, Vol. 8., 616200. doi: 10.3389/fmed.2021.616200.
- Kruger Ph., Saffarzadeh M., Weber A.N.R., Rieber N., Radsak M., von Bernuth H., Benarafa Ch., Roos D., Skokowa J., Hartl D. Neutrophils: between host defence, immune modulation, and tissue injury. PLoS Pathog., 2015, Vol. 11, no. 3, e1004651. doi: 10.1371/journal.ppat.1004651.
- Mantovani А., Cassatella M.C., Costantini C., Jaillon S. Neutrophils in the activation and regulation of innate and adaptive immunity. Nat. Rev. Immunol. 2011, Vol. 11, pp. 519-531.
- Marteyn B.S., Burgel P.R., Meijer L., Witko-Sarsat V. Harnessing neutrophil survival mechanisms during chronic infection by pseudomonas aeruginosa: novel therapeutic targets to dampen inflammation in cystic fibrosis. Front. Cell. Infect. Microbiol., 2017, Vol. 7, 243. doi: 10.3389/fcimb.2017.00243.
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