Co-cultivation of mastocytes and cumulus cells in polycystic ovary syndrome
- 作者: Valikova O.V.1,2,3, Zdor V.V.1,4, Tikhonov Y.N.1, Boroda A.V.5, Kolbin K.G.6
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隶属关系:
- Pacific State Medical University
- Regional Clinical Hospital No. 2
- Cliniс “Plastic Surgery”
- Cliniс for Diabetes and Endocrine Diseases
- A. Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, Russian Academy of Sciences
- Clinic for Reproduction and Genetics “Next Generation Clinic”
- 期: 卷 27, 编号 3 (2024)
- 页面: 483-492
- 栏目: SHORT COMMUNICATIONS
- URL: https://ogarev-online.ru/1028-7221/article/view/267514
- DOI: https://doi.org/10.46235/1028-7221-16802-CCO
- ID: 267514
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The purpose of the study was to study the cytokine-producing function of cumulus cells of the ovaries in PCOS during co-cultivation of cumulus cells with mast cells to identify their mutual influence in the immunopathogenesis of PCOS.
The study was approved by the Interdisciplinary Ethics Committee of the FSBEI of HE of the Ministry of Health of the Russian Federation. A permanent human mast cell line HMC-1 (Human mast cell, ATCC, USA) and a primary culture of cumulus cells were used. The condition of cumulus cells and mastocytes was determined using fluorescent dyes followed by flow cytometry before co-cultivation and after 7 days co-cultivation. The levels of IL-6, IL-10, and IFNã were studied on days 1, 3, and 7 of the experiment.
In monoculture and during co-culture of cells, the synthesis of cytokines IL-6, IL-10, and IFNã continues, but to varying degrees of severity. In the permanent mast cell line, an increase in IL-6, IL-10, and IFNã is observed on the 1st, 3rd, and 7th days of cultivation. On the first day, cytokine levels of donor cumulus cells and mastocytes did not differ significantly (p > 0.05). In the culture of donor cumulus cells, the synthesis of IL-6, IL-10, and IFNã progressively increases by the 7th day of the experiment (p < 0.05). On the first day of the experiment, the balance of Th1/Th2 cytokines in PCOS was twice as high as in healthy women. The ratio increased, and on the 7th day reached 3.83 times higher than the ratio of Th1/Th2 cytokines in the control group. Hyperproduction of IFNã by mastocytes was most significant when they were co-cultured with cumulus cells, in monoculture mast cells synthesized excessively cytokine twice from the initial values of IFNã, and the monoculture cumulus cells in PCOS it practically did not contain.
The studied cytokines are regulators of the function of ovarian cumulus cells and factors that increase the competence of the oocyte, indicating the need for their correction, which will allow a real influence on the links of pathogenesis in PCOS in the future.later on.
作者简介
O. Valikova
Pacific State Medical University; Regional Clinical Hospital No. 2; Cliniс “Plastic Surgery”
编辑信件的主要联系方式.
Email: renalex.99@mail.ru
Junior Research Associate, Endocrinologist
俄罗斯联邦, Vladivostok; Vladivostok; VladivostokV. Zdor
Pacific State Medical University; Cliniс for Diabetes and Endocrine Diseases
Email: renalex.99@mail.ru
PhD, MD (Medicine), Leading Research Associate, Endocrinologist
俄罗斯联邦, Vladivostok; VladivostokYa. Tikhonov
Pacific State Medical University
Email: renalex.99@mail.ru
Senior Lecturer, Department of Pathological Anatomy
俄罗斯联邦, VladivostokA. Boroda
A. Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, Russian Academy of Sciences
Email: renalex.99@mail.ru
PhD, Senior Research Associate, Laboratory of Cell Technology, Deputy Director for Scientific Activities
俄罗斯联邦, VladivostokK. Kolbin
Clinic for Reproduction and Genetics “Next Generation Clinic”
Email: renalex.99@mail.ru
PhD (Biology), Embryologist
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