THE STERIC AVAILABITY OF THYROID PEROXIDASE EPITOPES IN ENZYME IMMUNOASSAY FOR THE DETERMINATION OF AUTOANTIBODIES
- 作者: Kuzmina N.S.1, Zubkov A.V.1, Sviridov V.V.1
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隶属关系:
- Federal Statе Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
- 期: 卷 22, 编号 2-1 (2019)
- 页面: 356-358
- 栏目: ORIGINAL ARTICLES
- URL: https://ogarev-online.ru/1028-7221/article/view/119964
- DOI: https://doi.org/10.31857/S102872210006626-3
- ID: 119964
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全文:
详细
Evaluation of circulating autoantibodies (auto-At) to thyroid peroxidase (TPO) is widely used in the clinic to identify / confirm autoimmune diseases of the thyroid gland. Despite significant progress in creating tests for auto-At detection, the issues of their validation, standardization of methods attract close attention of both developers and users. Comparative studies of the level of antibodies in the blood serum of patients with diff use toxic goiter, autoimmune thyroiditis using diagnosticumof diff erent manufacturers showed that the discrepancies are associated with the TPO epitope structure immobilized on the solid phase. It is proposed to use monoclonal antibodies to control the antigenic structure of TPO as the process of isolation of the native protein, and the production of tests for the analysis of At to TPO.
作者简介
N. Kuzmina
Federal Statе Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
编辑信件的主要联系方式.
Email: ns_kuzim@mail.ru
Ph.D., leading research worker,
Moscow
俄罗斯联邦A. Zubkov
Federal Statе Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
Ph.D., Head of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
俄罗斯联邦V. Sviridov
Federal Statе Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
Ph.D., Head of the Laboratory of Cell Hybrids,
Moscow
俄罗斯联邦参考
- Czarnocka D., Eshler D. C., Godlewska, Nomer Y. Thyroid autoantibodies: thyroid peroxidase and thyroglobulin antibodies. In: Shoenfeld Y, Meroni PL, Gerswin MT (eds) Autoantibodies, 3rd edn. Elsevier, Amsterdam, 2014, 365–373.
- Tozzoli R., Villalta D., Bizzaro N. Challenges in the Standardization of Autoantibody Testing: a Comprehensive Review. Clin. Rev. Allergy Immunol., 2017, 53(1), 68–77.
- Кузьмина Н. С., Кузнецова Г. И., Яковлева И. В., Свиридов В. В., Буркин М. А., Мартынов А. И. Иммуноферментный метод определения аутоантител к пероксидазе щитовидной железы. Аллергия, астма и клиническая иммунология. 2000, 1, 135–137.
- Кузьмина Н.С., Яковлева И. В., Свиридов В. В., Зубков А. В., Кузнецова Г. И., Кириллова Г. А., Сергиенко О. В., Лунин В. Г., Буркин М. А., Летаров А. В., Лаврова Н. В., Рязанова Е. М. Моноклональные антитела к человеческой пероксидазе щитовидной железы. Биотехнология, 2005, № 1, 51–57
- Butler J. E., Navarro P., Sun J. Adsorption-induced antigenic changes and their significance in ELISA and immunological disorders. Immunol. Invest. 1997, 26(1–2), 39–54.
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