STEARYLAMINE ACTIVATES RAPID AND ROS-INDEPENDENT FORMATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETOSIS)
- 作者: Lotosh N.Y.1, Selischeva A.A.1,2, Vorobjeva N.V.3
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隶属关系:
- National Research Center «Kurchatov Institute»
- Lomonosov Moscow State University
- Lomonosov Moscow State University, Faculty of Biology
- 期: 卷 22, 编号 2-2 (2019)
- 页面: 855-857
- 栏目: ORIGINAL ARTICLES
- URL: https://ogarev-online.ru/1028-7221/article/view/119762
- DOI: https://doi.org/10.31857/S102872210006650-0
- ID: 119762
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详细
Using confocal fluorescence microscopy it was shown that stearylamine (SA) at concentration of 0.2 mg/ml causes the neutrophil extracellular traps formation. In comparison with classic NET activator, the phorbol ether of myristate acetate (PMA), SA activates this process much faster, in 30‒60 min, while under PMA stimulation it occurs in 2‒3 hours. Using luminol-dependent chemiluminescence, we showed that SA does not activate NADPH oxidase. SA dose-dependently suppresses the oxidative burst stimulated with PMA, zymosan, or latex. The direct evidence that NADPH oxidase is not activated under the SA action was obtained by using neutrophils isolated from the blood of patients with chronic granulomatous disease (CGD). It is obvious from our results, that SA-induced NET formation does not require the participation of reactive oxygen species and proceeds according to a nonclassical mechanism.
作者简介
N. Lotosh
National Research Center «Kurchatov Institute»
编辑信件的主要联系方式.
Email: natalotosh@gmail.com
Ph.D., Research Associate, Department of Biotechnology and Bioenergy,
Moscow
俄罗斯联邦A. Selischeva
National Research Center «Kurchatov Institute»;Lomonosov Moscow State University
Email: fake@neicon.ru
Doctor of Chemical Sciences, Research Engineer;
Leading Research Associate, Faculty of Biology,
Moscow
俄罗斯联邦N. Vorobjeva
Lomonosov Moscow State University, Faculty of Biology
Email: fake@neicon.ru
Ph.D., Senior Research Associate,
Moscow
俄罗斯联邦参考
- Воробьева Н. В., Пинегин Б. В. Нейтрофильные внеклеточные ловушки: механизмы образования, роль в норме и при патологии. Биохимия. 2014, 79 (12), 1580–1591.
- Fuchs T. A., Abed U., Goosmann C., Hurwitz R., Schulze I., Wahn V., Weinrauch Y., Brinkmann V., Zychlinsky A. Novel cell death program leads to neutrophil extracellular traps. J. Cell Biol, 2007, 176, 231–241.
- Pilsczek F. H., Salina D., Poon K. K., Fahey C., Yipp B. G., Sibley C. D., Robbins S. M., Green F. H., Surette M. G., Sugai M., Bowden M. G., Hussain M., Zhang K., Kubes P. J. A novel mechanism of rapid nuclear neutrophil extracellular trap formation in response to Staphylococcus aureus. J. Immunol., 2010, 185, 7413–7425.
- Лотош Н. Ю., Алясева С. О., Василов Р. Г., Селищева А. А. Стеариламин вызывает образование нейтрофильных внеклеточных ловушек независимо от активных форм кислорода. Цитология, 2019, 61(4), 1–10.
- Díaz-Godínez C., Fonseca Z., Néquiz M., Laclette J. P., Rosales C., Carrero J. C. Entamoeba histolytica Trophozoites induce a rapid non-classical NETosis. Mechanism independent of NOX2-derived reactive oxygen species and PAD4 activity. Front. Cell. Infect. Microbiol., 2018, 8, 184.
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