Experimental assessment of the immunotropic properties of the new biocomplund

The article concerns topical issues of studying the new biocompound within a consortium of biologically active substances (BAS). These compounds (metabiotics) are produced by probiotic strains 59T and 60T of saprophytic microorganisms which belong to Bacillus subtilis genus. These strains seem to be quite promising for the design of hepatopicts, the new drug candidates, for creation and development of a new class of hepatoprotector drugs. Previous studies have shown safety and hepatoprotective effects of abovementioned BAS. It is important to note that the combined use of these compounds provides a potentiated pharmacological effect. Moreover, there are significant reasons to believe that these BAS may influence different compartments of immune system. In this regard, it seemed appropriate to perform experimental assessment of the immunotropic effect of BAS in laboratory animals using a model of toxic liver damage with carbon tetrachloride, in order to confirm the effect on the humoral factors of immunity. The purpose of the present study is to provide experimental comparative assessment of humoral immunity indexes upon combined usage of biologically active substances in acute toxic liver damage. The experimental model of toxic damage was reproduced in white laboratory rats with repeated intragastric administration of carbon tetrachloride. The comparison drug was ursosan, a registered hepatoprotective drug. The following factors of humoral immunity were measured to assess the effects of biologically active substances: immunoglobulins M, G, A, quantitative indices of á-interferon and circulating immune complexes (CIC). As a result of the studies performed, we obtained reliable data showing increased amounts of all Ig classes as well as induction of endogenous á-interferon and antibody-forming cells under the conditions of acute toxic liver damage. Meanwhile, a significant restoration of the humoral immunity indices to initial values was registered after the 15-day treatment with biologically active substances. The data obtained allow us to recommend the studied BAS for creation of new drug candidates of microbial origin, hepatoprotective agents with immunotropic effects.