ANTI-TUMOR AND IMMUNOMODULATING EFFECTS OF RECOMBINANT HUMAN TUMOR NECROSIS FACTOR ALPHA IN A DELIVERY SYSTEMIN B16-F10 MELANOMA MICE
- Authors: Sysoeva G.M.1, Ryabchikova E.I.2, Gamaley S.G.1, Lebedev L.R.1, Volosnikova E.A.1, Danilenko E.D.1
-
Affiliations:
- Institute of Medical Biotechnology FBRI SRC VB «Vector» Rospotrebnadzor
- Institute of Chemical Biology and Fundamental Medicine SB RAS
- Issue: Vol 22, No 2-1 (2019)
- Pages: 575-577
- Section: ORIGINAL ARTICLES
- URL: https://ogarev-online.ru/1028-7221/article/view/120306
- DOI: https://doi.org/10.31857/S102872210006979-1
- ID: 120306
Cite item
Full Text
Abstract
It was established that recombinant human tumor necrosis factor alpha in a delivery system has pronounced antitumor and immunomodulating effects in murine B16-F10 melanoma model. The preparation significantly inhibited the growth of experimental tumor (TGI 60%), increased tumor infiltration by immune system cells (CD3+, CD11b+) and increased the activity of immunocompetent cells mediating antitumor response.
About the authors
G. M. Sysoeva
Institute of Medical Biotechnology FBRI SRC VB «Vector» Rospotrebnadzor
Author for correspondence.
Email: sysoeva_gm@vector.nsc.ru
SRF, Institute of Medical Biotechnology,
Berdsk, Novosibirsk Region
Russian FederationE. I. Ryabchikova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: fake@neicon.ru
Institute of Chemical Biology and Fundamental Medicine,
Berdsk, Novosibirsk Region
Russian FederationS. G. Gamaley
Institute of Medical Biotechnology FBRI SRC VB «Vector» Rospotrebnadzor
Email: fake@neicon.ru
Head of Department of Biological Research,
Berdsk, Novosibirsk Region
Russian FederationL. R. Lebedev
Institute of Medical Biotechnology FBRI SRC VB «Vector» Rospotrebnadzor
Email: fake@neicon.ru
Dr. habil., Head of the laboratory of Nucleic Acids and Recombinant Proteins,
Berdsk, Novosibirsk Region
Russian FederationE. A. Volosnikova
Institute of Medical Biotechnology FBRI SRC VB «Vector» Rospotrebnadzor
Email: fake@neicon.ru
PhD, Head of Laboratory of Obtaining and Analysis of Bio substances,
Berdsk, Novosibirsk Region
Russian FederationE. D. Danilenko
Institute of Medical Biotechnology FBRI SRC VB «Vector» Rospotrebnadzor
Email: fake@neicon.ru
PhD, director, Institute of Medical Biotechnology,
Berdsk, Novosibirsk Region
Russian FederationReferences
- Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth.1983; 65 (1–2): 55–63.
- Rook G. A. W., Steele J., Umar S., Dockrell H. M. A simple method for the solubilisation of reduced NBT, and its use as a colorimetric assay for activation of human macrophages by gamma-interferon. J. Immunol. Meth. 1985; 82 (1): 161–167.
- Kogan G., Sandula J., Korolenko T. A., Falameeva O. V., Poteryaeva O. N., Zhanaeva S. Y., Levina O. A., Filatova T. G., Kaledin V. I. Increased efficiency of Lewis lung carcinoma chemotherapy with a macrophage stimulator-yeast carboxymethyl glucan. Int. Immunopharmacol. 2002, 2; 6:775–781.
- Deryagina V. P., Ryzhova N. I., Krivosheeva L. V., Saluchinskay L. A., Golubeva I. S., Reutov V. P. Functional activity of effector cells of innate immunity in tumor bearing mice. Modern science, 2016, № 11, 69–76.
Supplementary files
