Genetic stability of the HA, NA, and NS genes of the recombinant vector virus FluNS1-124-Omp16 (H5N1) expressing the brucellar gene

S. O. Sadikaliyeva; Садикалиева С. О.; K. T. Sultankulova; Султанкулова К. Т.; K. A. Shorayeva; Шораева К. А.; V. M. Strochkov; Строчков В. М.; O. V. Chervyakova; Червякова О. В.; V. L. Zaitsev; Зайцев В. Л.; K. K. Tabynov; Табынов К. К.; N. T. Sandybayev; Сандыбаев Н. Т.; A. R. Sansyzbay; Сансызбай А. Р.; A. Yu. Egorov; Егоров А. Ю.

Genetic stability of the HA, NA, and NS genes of the recombinant vector virus FluNS1-124-Omp16 (H5N1) expressing the brucellar gene

Authors: Sadikaliyeva S.O.¹, Sultankulova K.T.¹, Shorayeva K.A.¹, Strochkov V.M.¹, Chervyakova O.V.¹, Zaitsev V.L.¹, Tabynov K.K.¹, Sandybayev N.T.¹, Sansyzbay A.R.¹, Egorov A.Y.²
Affiliations:
1. Scientific-Research Institute of Biological Safety Problems
2. HSC Development GmbH
Issue: Vol 60, No 4 (2015)
Pages: 18-23
Section: ORIGINAL RESEARCH
URL: https://ogarev-online.ru/0507-4088/article/view/118088
ID: 118088

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Abstract

The recombinant strain Flu-NS1-124-Omp16 (H5N1) of the influenza virus expressing the brucellar Omp16 gene was constructed on the basis of the technology of reverse genetics for the purpose of developing vector antibrucellosis vaccine. The obtained recombinant strain is a genetically stable construction. This stability is confirmed by the comparative analysis of the nucleotide sequences of the HA, NA, and NS genes of the recombinant vector virus Flu-NS1-124-Omp16 (H5N1) expressing the Omp16 gene of the Brucella abortus (genBank: AAA59360.1). The comparative analysis showed that the nucleotide sequence of the Ns gene of the first and the fifth passage level of the Flu-NS1-124-Omp16 (H5N1) virus corresponded for 100% to the initial part of 12ААS2TC_124Omp16g containing the chimera NS1-124-Omp16 in the composition of DNA (deoxyribonucleic acid) plasmids pHW2000. Total identity with HA and NA genes of the strain А/AstanaRG/6:2/2009 (H5N1) was shown by the comparative analysis of the nucleotide sequences of HA and NA genes of the first and the fifth passage level of the recombinant strain Flu-NS1-124-Omp16 (H5N1). The recombinant vector virus Flu-NS1-124-Omp16 (H5N1) expressing the brucella Omp16 gene maintains the genetic stability during 5 passages in 10-day developing chicken embryos.

Keywords

recombinant vector virus, brucellosis gene, hemagglutinin, neuraminidase, non-structural protein, PCR (polymerase chain reaction), genetic analysis

About the authors

S. O. Sadikaliyeva

Scientific-Research Institute of Biological Safety Problems

Author for correspondence.
Email: t.m.tlenchieva@mail.ru

Sandugash Sadikalieva, Junior research fellow

080409, Gvardeiskiy

Kazakhstan

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