Development of a Method for Determining the Amino Acid Composition of the Pharmaceutical Substance “Glatiramer Acetate” by NMR Spectroscopy

V. I. Krylov; A. A. Kutin; V. A. Yashkir; V. A. Merkulov; N. E. Kuz’mina; S. V. Moiseev

doi:10.1007/s11094-017-1587-2

Development of a Method for Determining the Amino Acid Composition of the Pharmaceutical Substance “Glatiramer Acetate” by NMR Spectroscopy

Авторы: Krylov V.I.¹, Kutin A.A.¹, Yashkir V.A.¹, Merkulov V.A.¹, Kuz’mina N.E.¹, Moiseev S.V.¹
Учреждения:
1. National Center for Expert Assessment of Medicines, “Russian Ministry of Health”
Выпуск: Том 51, № 3 (2017)
Страницы: 226-229
Раздел: Article
URL: https://ogarev-online.ru/0091-150X/article/view/244543
DOI: https://doi.org/10.1007/s11094-017-1587-2
ID: 244543

Цитировать

Полный текст

Открытый доступ
Доступ закрыт

Доступ предоставлен
Доступ закрыт

Только для подписчиков

Аннотация
Об авторах
Список литературы
Дополнительные файлы
Статистика

Аннотация

The acid hydrolysis procedure for glatiramer acetate was modified and the characteristic signals in the ¹H and ¹³C spectra were identified, allowing its amino acid composition to be determined. The relative molar contents of amino acids in glatiramer acetate measured by HPLC-MS and by ¹³C and ¹H NMR had close values. ¹³C NMR was found to be a more precise method for assaying the amino acid composition of glatiramer acetate than ¹H NMR.

Ключевые слова

glatiramer acetate, amino acid composition, acid hydrolysis, NMR spectroscopy