Method Development for Quantitative Determination of Ascorbic Acid by High-Performance Thin-Layer Chromatography
- Autores: Trineeva O.V.1, Safonova E.F.1, Slivkin A.I.1
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Afiliações:
- Voronezh State University
- Edição: Volume 51, Nº 10 (2018)
- Páginas: 938-944
- Seção: Article
- URL: https://ogarev-online.ru/0091-150X/article/view/244863
- DOI: https://doi.org/10.1007/s11094-018-1719-3
- ID: 244863
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Resumo
An economic and rapid procedure for identification and quantitative determination of ascorbic acid using high-performance thin-layer chromatography (HPTLC) was developed. The optimum conditions for chromatography of vitamin C on a thin layer of absorbent with quantitative interpretation of the HPTLC data were established experimentally and justified theoretically. The proposed procedure was validated using medicinal plant raw materials such as nettle leaves and sea-buckthorn fruit. The procedure could be used for quality control of substances, single-component and complex preparations, plant materials, biologically active supplements, premixes, and products of the food and cosmetics industries.
Sobre autores
O. Trineeva
Voronezh State University
Autor responsável pela correspondência
Email: trineevaov@mail.ru
Rússia, Voronezh, 394006
E. Safonova
Voronezh State University
Email: trineevaov@mail.ru
Rússia, Voronezh, 394006
A. Slivkin
Voronezh State University
Email: trineevaov@mail.ru
Rússia, Voronezh, 394006
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