Inhibitory Effect of Excoecaria Agallocha L. Extracts on Elastase and Collagenase and Identification of Metabolites Using HPLC-UV-MS Techniques

Abstract

The present study is aimed at evaluating the anti-elastase and anti-collagenase activity and determining the active constituents of Excoecaria agallocha L. plant extracts. Ethanol extracts of E. agallocha exhibit highest extraction yield (23%) and greater total content of phenolic compounds and flavonoids as compared to other extracts. At a concentration of 80 μg/mL, the ethanol extract produced more significant (p < 0.01) inhibition of elastase (82.7%) and collagenase (76.12%) activity and acted as a non-competitive inhibitor. Fourteen flavonoids were identified in the active ethanol extract. Further studies will provide direct way to the development of elastase and collagenase inhibitors from E. agallocha.