Optimization of the Conditions for Assay of Glutamic Acid by Thin Layer Chromatography

Abstract

We report here the selection and evidential basis of optimum conditions for the chromatography of glutamic acid in a thin layer of sorbent. The conditions for identifying and assaying this amino acid by high-performance thin layer chromatography on a personal computer using specialist software were optimized. The method was used to estimate glutamic acid contents in complexes of free amino acids in extracts from medicinal herb material (using sea buckthorne fruits and nettle leaves as examples).