Monitoring of Changes in Oxygen Concentration in Tissues by the Kinetics of Delayed Fluorescence of Exogenous Dyes


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Abstract

The dynamics of the consumption of oxygen during photodynamic processes and the subsequent restoration of its concentration in malignant tumors and healthy tissues of mice were studied in vitro by the kinetics of long-term luminescence of xanthene dyes. To assess changes in the oxygen concentration in tissues, delayed fluorescence can be used due to singlet–triplet annihilation of singlet oxygen and a sensitizer in the triplet state. Upon periodic pulse excitation of sensitizers, reversible quenching of delayed fluorescence was detected in tumors, which is associated with a decrease in the amount of oxygen in the tissues during photodynamic processes. A method for visualizing the restoration of the initial oxygen concentration in tissues is proposed.

About the authors

S. N. Letuta

Orenburg State University

Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

S. N. Pashkevich

Orenburg State University

Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

E. K. Alidzhanov

Orenburg State University

Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

Yu. D. Lantukh

Orenburg State University

Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

D. A. Razdobreev

Orenburg State University

Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

A. A. Chakak

Orenburg State University

Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

A. T. Ishemgulov

Orenburg State University

Author for correspondence.
Email: azamat.ischemgulov@yandex.ru
Russian Federation, Orenburg, 460018

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