Intracellular Localization and Phototoxicity Mechanisms of Chlorin e6 Derivatives and their Liposomal Formulations

Resumo

Abstract—Confocal microscopy and colocalization analysis using Pearson correlation coefficients were used to show that esterified chlorin e₆ derivatives and their liposomal forms are mainly localized in the endoplasmic reticulum, Golgi complexes, cell mitochondria, and levels of their localization in lysosomes are low. Cellular uptake and accumulation kinetics of chlorin e₆ derivatives were strongly depended on the type of pharmacological formulation used for photosesitizers administration, while intracellular localization was independent on the formulation. Differences in the photodynamic activity and sensitization mechanisms for chlorin e₆ derivatives and their liposomal forms were shown when compared to those of chlorin e₆ photosensitizers in K562 cells. It is assumed that the observed differences in the mechanisms of cellular damage are to a greater extent due to specific photosensitizer localization.