The Adhesiveness of the OmpF and OmpC Porins from Yersinia pseudotuberculosis to J774 Macrophages

Resumo

Abstract—The significance of the Yersinia pseudotuberculosis porins OmpF and OmpC for adhesion to macrophages J774 was assessed using optical trapping. The passive sensitization of polystyrene microspheres with the preparations of these porins was verified. Using a set of differently functionalized microspheres, it was shown that OmpF produced at a lower culture temperature contributes to the adhesiveness of the bacteria Y. pseudotuberculosis to macrophages, while OmpC synthesized at a temperature of 37°C does not have this property. These results can be explained by thermoinducible differences in the primary structure and conformational features of the outer loops of porin molecules. These differences are suggested to be necessary for effective circulation in the environment and manifestation of the invasive properties of Y. pseudotuberculosis as an agent of saprozoonotic infection.