Characterization of lipodisc nanoparticles containing sensory rhodopsin II and its cognate transducer from Natronomonas pharaonis

Resumo

We describe the preparation and properties of lipodisc nanoparticles–lipid membrane fragments with a diameter of about 10 nm, stabilized by amphiphilic synthetic polymer molecules. We used the lipodisc nanoparticles made of Escherichia coli polar lipids and compared lipodisc nanoparticles that contained the photosensitive protein complex of the sensory rhodopsin with its cognate transducer from the halobacterium Natronomonas pharaonis with empty lipodisc nanoparticles that contained no protein. The lipodisc nanoparticles were characterized by dynamic light scattering, transmission electron microscopy and atomic force microscopy. We found that the diameter of lipodisc nanoparticles was not affected by incorporation of the protein complexes, which makes them a prospective platform for single-molecule studies of membrane proteins.