The effect of synthetic antioxidants, anphen derivatives, on erythrocyte morphology

Abstract

The effect of new synthetic antioxidants, anphens, on erythrocyte morphology was studied. Insignificant cell transformations induced by the hydrophilic derivative of anphen-1 into echinocytes, as well as cell transformations into stomatocytes under the action of hydrophobic derivatives of anphens-2, 3, and 4 were revealed. The data we obtained indicate the intercalation of these compounds into the erythrocyte membrane. The distribution of compounds in the intra-membrane space depends on their hydrophobicity. A hydrophilic compound, anphen-1, is predominantly located in the outer monolayer of the membrane, while hydrophobic derivatives occur in the inner monolayer. It is proposed that the biological activities of anphen-3 and anphen-4 can occur in both monolayers as they move through the membrane, while the hydrophilic compound, anphen-1, exerts an insignificant membranotropic effect and can act only in the outer monolayer of the membrane. Variability in the efficiency of the concentration-dependent modifying action of the compounds with different hydrophobic properties has been found.