A study of the nature of plasma protein adsorption on the surface of perfluorocarbon emulsions stabilized by different triblock copolymers

Abstract

The composition of plasma proteins adsorbed on the surface of perfluorocarbon emulsions stabilized by different triblock copolymers and their quantitative ratio were analyzed. The results allowed us to describe three types of protein adsorption on the surface of emulsion droplets. Opsonin proteins prevailed during the first type of adsorption. Their adsorption occurred on a dense and inactive layer of triblock copolymers. The second type of adsorption occurred due to the hydrophobic effect on a dense and mobile layer, with low-molecular-weight proteins being predominantly adsorbed (monoadsorption). The third type of adsorption occurred on a loose layer of triblock copolymers. In this case, the adsorption of a large amount of proteins with a molecular weight of 10–500 kDa was observed, while the total molecular weight was distributed over a large number of proteins.