Email this article Magnetic isotope of magnesium accelerates ATP hydrolysis catalyzed by myosin
- Authors: Koltover V.K.1, Labyntseva R.D.2, Karandashev V.K.3, Kosterin S.O.2
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Affiliations:
- Institute of Problems of Chemical Physics
- Palladin Institute of Biochemistry
- Institute of Microelectronics Technology and High Purity Materials
- Issue: Vol 61, No 2 (2016)
- Pages: 200-206
- Section: Molecular Biophysics
- URL: https://ogarev-online.ru/0006-3509/article/view/151943
- DOI: https://doi.org/10.1134/S0006350916020068
- ID: 151943
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Abstract
In this paper, we present the results of experimental studies on the influence of different magnesium isotopes, magnetic 25Mg and nonmagnetic 24Mg or 26Mg, on ATP-hydrolytic activity of the isolated myosin subfragment-1. The reaction rate in the presence of magnetic 25Mg isotope turned out to be 2.0–2.5 times higher than that using non-magnetic 24Mg or 26Mg isotopes. In absence of the enzyme, as at spontaneous ATP hydrolysis in aqueous solution, no magnetic isotope effect was observed. Thus, a significant catalytic effect of the magnetic 25Mg isotope (nuclear spin catalysis) was discovered in the enzymatic hydrolysis of ATP.
About the authors
V. K. Koltover
Institute of Problems of Chemical Physics
Author for correspondence.
Email: koltover@icp.ac.ru
Russian Federation, pr. Akademika Semenova 1, Chernogolovka, Moscow oblast, 142432
R. D. Labyntseva
Palladin Institute of Biochemistry
Email: kinet@biochem.kiev.ua
Ukraine, ul. Leontovicha 9, Kyiv, 01601
V. K. Karandashev
Institute of Microelectronics Technology and High Purity Materials
Email: kinet@biochem.kiev.ua
Russian Federation, ul. Akademika Ossipyana 6, Chernogolovka, Moscow oblast, 142432
S. O. Kosterin
Palladin Institute of Biochemistry
Author for correspondence.
Email: kinet@biochem.kiev.ua
Ukraine, ul. Leontovicha 9, Kyiv, 01601
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