Peculiarities of the interaction between vincristine and blood serum

Abstract

The results of a study of the interaction between the pharmaceutical anticancer drug vincristine and blood serum are presented. It is shown that an increase in drug concentration is associated with a concomitant decrease in the extinction coefficient of the solute from blood serum. As the drug concentration changes, its fluorescence spectra transform; the fluorescence maximum of vincristine in blood serum is shifted to 314 and 334 nm. This can be the result of conformational changes in the vincristine molecule after the vincristine–blood protein interaction. At the same time, the fluorescence maximum at 360 nm, which can be explained by the fluorescence emission of the aromatic ring of vincristine, remains at the same level after an increase in the drug dose. These results can be useful in medicine for further investigation of the antimitotic activity of vincristine and test dosing of other pharmacological preparations during therapy.